Antibodies for
Immunohistochemistry
KBA.62 (KBA.62)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45319 IMPATH KBA.62 RTU M (KBA.62)
50 Tests
44335 KBA.62 RTU M (KBA.62)
7 ml Ready To Use
44690 KBA.62 0,1 M (KBA.62)
100 µl liquid Concentrated
44691 KBA.62 1 M (KBA.62)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Melanoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP 2-step Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-KBA.62 (Melanoma Associated Antigen) is a novel anti-melanoma antibody. Studies thus far have shown a similar sensitivity to melanocytic
proliferations as that seen with S-100 protein staining, which is somewhat higher than that seen with anti-HMB-45. This has been confirmed by
one study on a series of 215 sentinel lymph nodes. Moreover, anti-KBA.62 identified 6 patients (3%) who had confirmed sentinel lymph node
metastasis but stained negative using anti-HMB-45. In this setting, the resolution appears to be higher than S-100 protein in that the staining
pattern (membranous) is quite distinct. Interestingly, most cases of desmoplastic and spindle cell melanomas show strongly positive results with
anti-KBA.62, unlike that seen with other melanocyte markers. It should be noted that anti-KBA.62 will label occasional endothelial cells which
can serve as an internal positive control. A small percentage of well-differentiated squamous cell carcinomas of the skin (and lung) have also
been noted to stain with this antibody; however, the poorly-differentiated forms of carcinoma do not, thus resolving a greater practical problem in
differential diagnosis. Anti-KBA.62 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context
of micrometastasis in sentinel lymph node.
KBA.62 S-100 HMB-45 MART-1 Tyrosinase MiTF
CD63 Factor
XIIIa
WT1
PNL2
Adult Melanocytes
+
+
-
+
+
+
+
-
+
Junctional Nevus
+
+
+
+
+
+
-
-
+/-
+
Interdermal Nevus
+
+
-
+
+
+
-
-
+/-
+
Primary Melanoma
+
+
+
+
+
+
+
-
+
Metastatic Melanoma
+
+
+
+
+
+
+
-
+
+
Spindle Cell Melanoma
+
+
+
+
+
+
+
-
+
+
Angiomyolipoma
-
+
+
+
-
+
+
-
+
Adrenal Cortical Lesions
-
+
-
+
-
-
-
-
-
Intranodal Nevus Cells
+
+
-
+
+
+
-
-
+
Dermatofibroma
-
-
-
-
-
-
-
+
-
Reference
1. Kocan P, et al. Cesk Patol. 2004 Apr; 40(2):50-6.
2. Cecile Pages, et al. Hum Pathol. 2008; 39:1136-1142.
3. E Cohen-Knafo, et al. J Clin Pathol. 1995; 48:826-831.
4. Kaufmann O, et al. Mod Pathol. 1998 Aug; 11(8):740-6.
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