Antibodies for
Immunohistochemistry
LH (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45224 IMPATH LH RTU R (Poly)
50 Tests
44328 LH RTU R (Poly)
7 ml Ready To Use
44678 LH 0,1 R (Poly)
100 µl liquid Concentrated
44679 LH 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Pituitary
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Luteinizing hormone (LH) is a glycoprotein. Each monomeric unit is a sugar-like protein molecule; two of these make the full, functional protein.
Its structure is similar to the other glycoproteins, follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic
gonadotropin (hCG). The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits that are connected by two bridges. The
alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. LH has a beta subunit of 121 amino
acids (LHB) that confers its specific biologic action and is responsible for interaction with the LH receptor. This beta subunit contains the same
amino acids in sequence as the beta subunit of hCG and both stimulate the same receptor; however, the hCG beta subunit contains an additional
24 amino acids and the hormones differ in the composition of their sugar moieties. The gene for the alpha subunit is located at chromosome
6q12.21. The luteinizing hormone beta subunit gene is localized in the LHB/CGB gene cluster at chromosome 19q13.32. In contrast to the alpha
gene activity, beta LH subunit gene activity is restricted to the pituitary gonadotropic cells. It is regulated by the gonadotropin releasing hormone
from the hypothalamus. Inhibin, activin, and sex hormones do not affect genetic activity for the beta subunit production of LH. In both males
and females, LH is essential for reproduction. Mutations in this gene are associated with hypogonadism which is characterized by infertility and
pseudohermaphroditism.
Anti-LH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with LH-producing cells (gonadotrophs).
Pituitary Panel
LH
ACTH
FSH
GH
Prolactin
TSH
Pituitary
+
+
+
+
+
+
Reference
1. La Rosa S, et al. Virchows Arch. 2000 Sep; 437(3):264-9.
2. Saccomanno K, et al. J Clin Endocrinol Metab. 1994 May; 78(5):1103-7.
3. Felix I, et al. Hum Pathol. 1991 Jul; 22(7):719-21.
4. Sano T, et al. Virchows Arch A Pathol Anat Histopathol. 1990; 417(4):361-7.
158
