Advanced Solutions
for Advanced Pathology
Kappa (L1C1)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
44323 Kappa Light Chain RTU M (L1C1)
7 ml Ready To Use
44668 Kappa Light Chain 0,1 M (L1C1)
100 µl liquid Concentrated
44669 Kappa Light Chain 1 M (L1C1)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
Anti-kappa detects surface immunoglobulin on normal and neoplastic B-cells. In paraffin-embedded tissue, anti-kappa exhibits strong
staining of kappa-positive plasma cells and cells that have absorbed exogenous immunoglobulins. When dealing with B-cell neoplasms, the
determination of light chain ratios remains the centerpiece. Most B-cell lymphomas express either kappa or lambda light chains, whereas
reactive proliferations display a mixture of kappa and lambda positive cells. If only a single light chain type is detected, a lymphoproliferative
disorder exists. Monoclonality is determined by a kappalambda ratio of greater than 3:1 or a lambda-kappa ratio greater than 2:1.
Immunoglobulin, Heavy and Light Chain
Kappa
IgA
IgG
IgD
IgM
Lambda
Myeloma
+/-
+
+
-/+
-/+
-/+
Diffuse LBCL
+/-
-
+
-
+
-/+
Marginal Zone Lymphoma
+/-
-
-
-/+
+
-/+
SLL/CLL
+/-
-
-
+
+
-/+
B-cell Lymphomas
Kappa Lambda CD79a BCL2
BCL6
CD5
CD10
CD23 Cyclin D1 Annexin
A1
Follicular
+/-
-/+
+
+
+
-
+
-
-
-
CLL/SLL
+/-
-/+
+
+
-
+
-
+
-
-
Mantle Cell
+/-
-/+
+
+
-
+
-
-
+
-
Marginal Zone
+/-
-/+
+
+
-
-
-
-
-
-
Lymphoplasmacytic
+/-
-/+
+
+
-
-
-
-
-
-
Diffuse Large Cell
+/-
-/+
+
+
+
-/+
-/+
-
-
-
Burkitt
+/-
-/+
+
-
+
-
+
-
-
-
Hairy Cell Leukemia
+/-
-/+
+
+
-
-
-
-
+(weak)/-
+
Reference
1. Michie SA, et al. A J Clin Path. 1987.
2. Hertel BF, et al. Lab Invest. 1977; 36:12.
3. Taylor CL. Arch Pathol Lab Med. 1978; 12:113-121.
4. Ashton-Key M, et al. Histopathology. 1996 Dec; 29(6):525-31.
5. Kurtin PJ, et al. Am J Clin Pathol. 1999 Sep; 112(3):319-29.
6. Abbondanzo SL, et al. Ann Diagn Pathol. 1999 Oct; 3(5):318-27.
7. Lee LA, et al. Am J Otolaryngol. 2002 Sep-Oct; 23(5):316-20.
8. Mendes S, Dreno B. Acta Derm Venereol. 2003; 83(3):167-70.
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