Antibodies for
Immunohistochemistry
MUC1 (MRQ-17)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45230 IMPATH MUC1 RTU M (MRQ-17)
50 Tests
44340 MUC1 RTU M (MRQ-17)
7 ml Ready To Use
44700 MUC1 0,1 M (MRQ-17)
100 µl liquid Concentrated
44701 MUC1 1 M (MRQ-17)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Associated adenocarcinomas,
Breast, Colon
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer that protects the gastric epithelium.
The heterogeneous pattern of mucin expression, including the expression of the intestinal mucin MUC2, may provide new insights into the
differentiation pathways of gastric carcinoma. Pinto-de-Sousa et al. Have shown, in a comprehensive study of gastric carcinomas evaluated
for expression of several mucins (MUC1, MUC2, MUC5AC, and, MUC6), that: (1) mucin expression is associated with tumor type (MUC5AC
with diffuse and infiltrative carcinomas and MUC2 with mucinous carcinomas) but not with the clinico-biological behavior of the tumors; and (2)
mucin expression is associated with tumor location (MUC5AC with antrum carcinomas and MUC2 with cardia carcinomas), indirectly reflecting
differences in tumor differentiation according to tumor location.
The following generalities apply to the patterns of MUC1 expression: Apical surfaces of most epithelial cells in breast, GI, respiratory, and GU
tracts.
Mucin Expression in Neoplasms
MUC1
MUC2
MUC5AC
MUC6
Pancreatic
Adenocarcinoma
+
-
+
-
Cervical Adenocarcinoma
+
-
+
-
Paget Extramammary
+
-
+
-
Cholangiocarcinoma
+
-
+/-
-
Breast Carcinoma
+
-
-
-
Endometrial Carcinoma
+
-
-
-
Barrett Esophagus
+
+
+
-
Breast Colloid Carcinoma
+
+
-
+
Mucins Expression in Organs
MUC1
MUC2
MUC4
MUC5AC
MUC6
Stomach
+
-
+
+
+
Small Intestine
-
+
-
-
+
Colon
-
+
-
-
Pancreas
+
-
-
-
+
Reference
1. Chaves P, et al. Dis Esophagus. 2005; 18(6):383-7.
2. Leteurtre E, et al. World J Gastroenterol. 2006 Jun 7; 12(21):3324-31.
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