Antibodies for
Immunohistochemistry
Microphthalmia Transcription Factor (MiTF) (C5/D5)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45226 IMPATH MITF RTU M (C5/D5)
50 Tests
44336 MITF RTU M (C5/D5)
7 ml Ready To Use
44692 MITF 0,1 M (C5/D5)
100 µl liquid Concentrated
44693 MITF 1 M (C5/D5)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Melanoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Microphthalmia (MiTF) is a transcription factor implicated in pigmentation, bone development, and in mast cells. Various forms of MiTF exist
ranging from 50 -70 kD in size. This antibody targets the 52 - 56 kD range and has been useful in identifying malignant melanoma and distinguishing
mast cell lesions from lesions of myeloid derivation. A relatively rare class of tumors known as PEComas (tumors showing perivascular epithelioid
cell differentiation) express MiTF in a high percentage of cases (~90%).
Melanotic Lesions
MiTF
S-100
SOX-10
HMB-45
MART-1 Tyrosinase
CD63
Factor XIIIa
Adult Melanocytes
+
+
+
-
+
+
+
-
Junctional Nevus
+
+
+
+
+
+
-
-
Interdermal Nevus
+
+
+
-
+
+
-
-
Primary Melanoma
+
+
+
+
+
+
+
-
Metastatic Melanoma
+
+
+
+
+
+
+
-
Spindle Cell Melanoma
+
+
+
+
+
+
+
-
Angiomyolipoma
+
+
+
+
+
-
+
-
Adrenal Cortical
-
+
-
+
-
-
-
Intranodal Nevus Cells
+
+
+
-
+
+
-
-
Dermatofibroma
-
-
-
-
-
-
-
+
Reference
1. Liegl B, et al. Am J Surg Pathol. 2008 Apr; 32(4):608-14.
2. Righi A, et al. Int J Surg Pathol. 2008 Jan; 16(1):16-20.
3. Weinreb I, et al. Virchows Arch. 2007 Apr; 450(4):463-70. Epub 2007 Feb 15.
4. Ohsie SJ, et al, Binder SW. J Cutan Pathol. 2008 May; 35(5):433-44. Review.
5. Hornick JL, Fletcher CD. Am J Surg Pathol. 2008 Apr; 32(4):493-501.
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