Advanced Solutions
for Advanced Pathology
MUC2 (MRQ-18)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45231 IMPATH MUC2 RTU M (MRQ-18)
50 Tests
44341 MUC2 RTU M (MRQ-18)
7 ml Ready To Use
44702 MUC2 0,1 M (MRQ-18)
100 µl liquid Concentrated
44703 MUC2 1 M (MRQ-18)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Colon
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP 2-step Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer that protects the gastric epithelium.
The heterogeneous pattern of mucin expression, including the expression of the intestinal mucin MUC2, may provide new insights into the
differentiation pathways of gastric carcinoma. Pinto-de-Sousa et al. have shown, in a comprehensive study of gastric carcinomas evaluated
for expression of several mucins (MUC1, MUC2, MUC5AC and MUC6), that: (1) mucin expression is associated with tumor type (MUC5AC
with diffuse and infiltrative carcinomas and MUC2 with mucinous carcinomas) but not with the clinico-biological behavior of the tumors; and (2)
mucin expression is associated with tumor location (MUC5AC with antrum carcinomas and MUC2 with cardia carcinomas), indirectly reflecting
differences in tumor differentiation according to tumor location. The following generalities apply to the patterns of MUC2 expression: Specifically
expressed in goblet cells of the small intestine & colon; colonic carcinomas – 65%, gastric carcinomas – 42%. MUC2 is rarely expressed outside
of the GI tract with exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.
Mucin Expression in Neoplasms
MUC2
MUC1
MUC5AC
MUC6
Salivary Duct ACA
+
-
-
+
Colon Carcinoma, Signet
Ring
+
-
-
-
Prostate Carcinoma
+/-
-
-
-
Pan Intraductal Pap Ca
+
-
+
+
Adrenocortical Carcinoma
-
-
-
-
Breast Carcinoma
-
+
-
-
Endometrial Carcinoma
-
+
-
-
Appendiceal
Adenocarcinoma
+
-
+
-
Barrett Esophagus
+
+
+
-
Breast Colloid Carcinoma
+
+
-
+
Mucins Expression in Organs
MUC2
MUC1
MUC4
MUC5AC
MUC6
Stomach
-
+
+
+
+
Small Intestine
+
-
-
-
+
Colon
+
-
-
-
Pancreas
-
+
-
-
+
Reference
1. Chaves P, et al. Dis Esophagus. 2005; 18(6):383-7.
2. Leteurtre E, et al. World J Gastroenterol. 2006 Jun 7; 12(21):3324-31.
169
