Antibodies for
Immunohistochemistry
Lysozyme (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45151 IMPATH Lysozyme RTU R (Poly)
50 Tests
44329 Lysozyme RTU R (Poly)
7 ml Ready To Use
44680 Lysozyme 0,1 R (Poly)
100 µl liquid Concentrated
44681 Lysozyme 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-lysozyme stains myeloid cells, histiocytes, granulocytes, macrophages, and monocytes. It is an important marker that may demonstrate
the myeloid or monocytic nature of acute leukemia. The restrictive nature of anti-lysozyme staining suggests that lysozyme may be synthesized
predominantly in reactive histiocytes rather than in resting, unstimulated phagocytes. Anti-lysozyme may aid in the identification of histiocytic
neoplasias, large lymphocytes, and classifying lymphoproliferative disorders.
Acute Myeloid Leukemia
Lysozyme
MPO CD68 Factor VIII
CD61
BOB.1
Oct-2
Glyco-
phorin A CD34
Acute Myeloid, M0
+
-
-
-
-
-
-
-
+
Acute Myeloid, M1&2
+
+
+
-
-
-
+
Promyelocytic, M3
-
+
-
-
-
+
+
-
-
Myelomonocytic, M4
+
+
+
-
-
-
+
-
+
Monoblastic, M5
+
+
+
-
-
-
+
-
-/+
Acute Erythroid, M6
+
-
-
-
-
-
+
-/+
Megakaryoblastic, M7
-
-
+
+
+/-
-
-
-
Lymph Node
Lysozyme
CD68
S-100
CD1a
CD21/CD35
PD-1
Reactive Histiocytosis
+
+
-
-
-
-
Langerhans Cell
Histiocytosis
+
+
+
+
-
-
Sinus Histiocytosis with
Massive Lymphadenopathy
+
+
+
-
-
-
Follicular Dendritic Cell
Sarcoma
-
-
-
+/-
+
-
Dermatopathic
Lymphadenitis
+
-
+
+
-
-
Histiocytic Lesions
Lysozyme
CD45
CD4
CD68
CD163 Factor XIIIa
CD20
CD3
Histiocytic Lesions
+
+
+
+
+
+
-
-
Reference
1. Morsky P. Clin Chim Acta. 1988; 178:327-36.
160
