Antibodies for
Immunohistochemistry
Insulin (Polyclonal)
Guinea Pig Polyclonal Antibody
Cat. No. Description
Volume
45222 IMPATH Insulin RTU G (Poly)
50 Tests
44322 Insulin RTU G (Poly)
7 ml Ready To Use
44666 Insulin 0,1 G (Poly)
100 µl liquid Concentrated
44667 Insulin 1 G (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Pancreas
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Insulin is a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Insulin is one of the major regulatory
hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein,
and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage,
formation of triglycerides, and synthesis of proteins and nucleic acids. Immunohistochemical investigations have localized insulin in the beta
cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in
the general population. Insulin is also present in tumors of beta cell origin such as insulinoma. Anti-insulin staining in the cytoplasm of tumors is
the most reliable indication of functional insulinomas.
Pancreas
Insulin Synapto-
physin
Chromo-
granin A Gastrin CD56 β-Catenin CK 19 CA19-9 E-cadherin CD10
Neuroendocrine Tumor
+/-
+
+
+/-
+
+
+/-
+/-
-
-
Solid Pseudopapillary
Tumor
-
+
-
-
+
+
-
-
+(nuclear)
+
Ductal Carcinoma
-
-
-
-
-
+/-
+
+
+/-
+/-
Acinic Cell Carcinoma
-
-
-
-
-
+
+
-/+
+
+/-
Pancreatoblastoma
-
-
+
-
+
+
-
-
-
-
Normal Pancreas
+
+
+
-
-
+
-
-
-
-
Reference
1. Akagi T, et al. Cancer. 1981; 47:417-424.
2. Scully RE, et al. N eng J Med. 1983; 308:30-37.
3. Erlandsen SL. Williams and Wilkins, Baltimore. 1980; 140-155.
4. Friesen SR. N Eng J Med. 1982; 306:580-590.
5. Jorda M, et al. Arch Pathol Lab Med. 2003 Feb; 127(2):196-9.
6. Letizia C, et al. Eur J Endocrinol. 2001 May; 144(5):517-20.
7. Govindarajan M, et al. Diabetes Res Clin Pract. 2001 Jan; 51(1):29-38.
8. Azzoni C, et al. Virchows Arch. 1998 Dec; 433(6):495-504.
9. Lubensky IA, et al. Am J Pathol. 1998 Jul; 153(1):223-31.
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