Antibodies for
Immunohistochemistry
Myogenin (F5D)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45238 IMPATH Myogenin RTU M (F5D)
50 Tests
44348 Myogenin RTU M (F5D)
7 ml Ready To Use
44716 Myogenin 0,1 M (F5D)
100 µl liquid Concentrated
44717 Myogenin 1 M (F5D)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Rhabdomyosarcoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some
leiomyosarcomas. Positive nuclear staining may occur in Wilms’ tumor.
Small, Round Blue Cell Tumors
Myogenin MS Actin SM Actin CD45
CK
Cocktail
CD99 PGP 9.5 FLI-1 Vimentin CD57
Lymphoblastic Lymphoma
-
-
-
+
-
-
+
+
-
Leiomyosarcoma
-
+
+
-
-/+
-
-
-
+
+/-
Rhabdomyosarcoma
+
+
-
-
-
-
+
-
+
-
Neuroblastoma
-
-
-
-
-
-
+
-
+
+
Embryonal Carcinoma
-
-
-
-
+
-
+
-
-
+
PNET/ES
-
-
-
-
-/+
+
+
+
+
+
DSRCT
-
-
-
-
+
-
-
+
+
+/-
Medulloblastoma
-
-
-
-
-
-
-
-
+
Spindle Cell Tumors
Myogenin β-Catenin PGP 9.5 CD117
ALK CD34
CK
Cocktail
Calponin MS Actin CD56
Myofibroblastic Tumor
-
-
-
-
+
-
-
+
+
+
Spindle Cell Carcinoma
-
+/-
+
-
-
-
+
-
-
-
Neurofibroma
-
-
+
-
-
-
-
-
-
+
Rhabdomyosarcoma
+
-
-
+
-
-
-
-
+
-
Endometrial Stromal Tumor
-
+/-
+
-
-
-
-
+
+
-
Smooth Muscle
-
-
-
-
-
-
-
+
+
-
Fibromatosis
-
+
+
-
-
-
-
-
-
-
GIST
-
-
-
+
-
+
-
-
-
-
Schwannoma
-
-
-
-
-
-
-
-
-
+
Leiomyosarcoma
+/-
-
-
-
-
-
-/+
+
+
+
Reference
1. Miller JB. J Cell Biol. 1990 Sep; 111(3):1149-59.
2. Wang NP, et al. Am J Pathol. 1995 Dec; 147(6):1799-810.
3. Cui S, et al. Pathol Int. 1999 Jan; 49(1):62-8.
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