Advanced Solutions
for Advanced Pathology
Myoglobin (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45239 IMPATH Myoglobin RTU R (Poly)
50 Tests
44349 Myoglobin RTU R (Poly)
7 ml Ready To Use
44718 Myoglobin 0,1 R (Poly)
100 µl liquid Concentrated
44719 Myoglobin 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Skeletal Muscle
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Immunostaining with anti-myoglobin provides a specific, sensitive, and practical procedure for the identification of tumors of muscle origin.
Since myoglobin is found exclusively in skeletal and cardiac muscle and is not present in any other cells of the human body, it may be used to
distinguish rhabdomyosarcoma from other soft tissue tumors. Anti-myoglobin staining is also useful when demonstrating rhabdomyoblastic
differentiation in other tumors, e.g. neurogenic sarcomas and malignant mixed mesodermal tumors of the uterus and ovary.
Small, Round Blue Cell Tumors
Myoglobin MS Actin SM Actin Myogenin CK
Cocktail
CD99
FLI-1
INI-1
CD57 PGP 9.5
Lymphoblastic Lymphoma
-
-
-
-
-
+
+
+
-
Rhabdomyosarcoma
+
-/+
-/+
+
-
-
-
+
-
+
Neuroblastoma
-
-
-
-
-
-
-
+
+
+
Embryonal Carcinoma
-
-
-
-
+
-
-
+
+
+
PNET/ES
-
-
-
-
-/+
+
+
+
+
+
DSRCT
-
-
-
-
+
-
+
+
+/-
-
Medulloblastoma
-
-
-
-
-
-
-
+
+
Reference
1. Furlong MA, et al. Mod Pathol. 2001 Jun; 14(6):595-603.
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