Antibodies for
Immunohistochemistry
CD20 (L26)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45128 IMPATH CD20 RTU M (L26)
50 Tests
44227 CD20 RTU M (L26)
7 ml Ready To Use
44476 CD20 0,1 M (L26)
100 µl liquid Concentrated
44477 CD20 1 M (L26)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Lymph Node, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2a
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-CD20 (Pan-B-cell) reacts with a membrane antigen of B-cells. This antibody also recognizes Reed-Sternberg cells, in classic Hodgkin
lymphoma, in about 20% to 40% of cases. CD20 has occasionally been detected in T-cell malignancies. However, it is a very strong marker of
mature B-cell lymphomas. Anti-CD20 does not usually immunoreact with non-hematopoietic neoplasms.
B-cell Lymphomas
CD20
BCL2 CD79a
IgD BCL6 Annexin
A1
CD10
CD23 Cyclin D1 MUM1
Follicular
+
+
+
+
+
-
+
-
-
-
CLL/SLL
+
+
+
+
-
-
-
+
-
+
Mantle Cell
+
+
+
+
-
-
-
-
+
-/+
Marginal Zone
+
+
+
-/+
-
-
-
-
-
+
Lymphoplasmacytic
+
+
+
-
-
-
-
-
-
+
Diffuse Large Cell
+
+
+
-
+
-
-/+
-
-
+
Burkitt
+
-
+
-
+
-
+
-
-
-
Hairy Cell Leukemia
+
+
+
-
-
+
-
-
+(weak)/-
Lymphoblastic Lymphomas, B-cell vs. T-cell
CD20
TdT
CD10
PAX-5
CD19
CD3
CD5
CD7
CD117 CD1a
B-cell
+/-
+
+
+
+
-
-
-
-
+
T-cell
-
+
+/-
-
-
+
+/-
+/-
-/+
+/-
Reference
1. Ishii Y, et al. Clin Exp Immuno. 1984; 58:183-192.
2. Davey FR, et al. Am J Pathol. 1987; 129:54-63.
3. Mason DY. Am J Pathol. 1987; 128:1-4.
4. Browne P, et al. Am J Clin Pathol. 2003 Nov; 120(5):767-777.
5. Tzankov A, et al. Clin Cancer Res. 2003 Apr; 9(4):1381-6.
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