ImPath - page 136

Antibodies for
Immunohistochemistry
Hepatitis B Virus Surface Antigen (S1-210)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45213 IMPATH Hepatitis B Virus Surface RTU M (S1-210) RUO 50 Tests
44305 Hepatitis B Virus Surface Anti RTU M (S1-210) RUO 7 ml Ready To Use
44632 Hepatitis B Virus Surface Anti 0,1 M (S1-210) RUO 100 µl liquid Concentrated
44633 Hepatitis B Virus Surface Anti 1 M (S1-210) RUO
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Hepatitis B infected tissue
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2b
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-hepatitis B surface antigen labels the cell cytoplasm infected with hepatitis B virus, a common cause of hepatitis leading to cirrhosis.
Hepatitis B is the second most common cause of parenterally transmitted hepatitis.
Reference
1. Stahl S, et al. Proc Natl Acad Sci. 1982; 79:1606.
2. Goodman ZD, et al. Am J Clin Pathol. 1988 Apr; 89(4):533-7.
3. van den Oord JJ, et al. J Histochem Cytochem. 1989 Apr; 37(4):551-4.
4. Sharma RR, et al. Trop Gastroenterol. 2002 Jan-Mar; 23(1):16-9.
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