Advanced Solutions
for Advanced Pathology
INI-1 (MRQ-27)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
44321 INI-1 RTU M (MRQ-27)
7 ml Ready To Use
44664 INI-1 0,1 M (MRQ-27)
100 µl liquid Concentrated
44665 INI-1 1 M (MRQ-27)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Astrocytoma, Brain, Endothelial cells
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2a
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
The INI-1 gene, which encodes a functionally uncharacterized protein component of the hSWI/SNF chromatin remodeling complex, is often
mutated or deleted in malignant rhabdoid tumor (MRT). Two isoforms of INI-1, that differ by the variable inclusion of amino acids, potentially
are produced by differential RNA splicing. The morphology of MRTs can present challenges in differential diagnosis. The overall survival of
patients with MRT relative to its potential mimics (medulloblastoma, supratenorial primitive neuroectodermal tumors (sPNETs)) is quite low,
and thus differentiation from these other tumors is desirable. Lack of nuclear labeling by anti-INI-1 is characteristic of MRT. The majority of
medulloblastomas and sPNETs are labeled by anti-INI-1. Epithelioid sarcoma in soft tissue frequently demonstrates rhabdoid features in
histology. Sometimes, it is difficult to distinguish epithelioid sarcoma from malignant rhabdoid tumor since both tumors have demonstrated a
loss of INI-1 expression. However, epithelioid sarcoma shows anti-CD34 and anti-β-Catenin immunoreactivity. MRT is negative for both markers.
Small, Round Blue Cell Tumors
INI-1 MS Actin Myoglobin Myogenin CK
Cocktail
CD99
CD57
FLI-1
WT1 Vimentin
Rhabdomyosarcoma
+
+
+
+
-
-
-
-
-
+
PNET/ES
+
-
-
-
-/+
+
+
+
-
+
DSRCT
+
-
-
-
+
-
+/-
+
+
+
Medulloblastoma
+
-
-
-
-
+
-
-
Brain: CNS Tumors
INI-1
GFAP Neuro-
filament
Synapto-
physin S-100
CK
Cocktail
PR
EMA Vimentin NGFR
Astrocytoma
+
+
-
-
+
-
-
-
+
+
Glioblastoma
+
+
-
-
+
-
-
-
+
-
Oligodendriglioma
+
-
-
-
+
-
-
-
+
-
Ependymoma
+
+
-
-
+
-
-
-
-/+
+
Choroid Plexus Carcinoma
+
-
-
-
+
+
-
-
-
Neuroblastoma
+
+/-
+
+
+/-
-
-
-
+
+
Pineocytoma
+
-
-
+
-
-
-
-
-
Meningioma
+
-
-
-
-
-
+
+
+
-
Rhabdoid Tumors
-
+/-
+/-
+/-
+/-
+
+
+
Reference
1. Bourdeaut F, et al. J Pathol. 2007 Feb; 211(3):323-30.
2. Fowler DJ, et al. Fetal PediatrPathol. 2006 May-Jun; 25(3):159-68.
3. Haberler, C et al. Am J SurgPathol. 2006 Nov; 30(11):1462-8.
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