Advanced Solutions
for Advanced Pathology
IgM (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45220 IMPATH IgM RTU R (Poly)
50 Tests
44319 IgM RTU R (Poly)
7 ml Ready To Use
44660 IgM 0,1 R (Poly)
100 µl liquid Concentrated
44661 IgM 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Immunoglobulin M (IgM) is a basic antibody that is present on B-cells. It is the primary antibody against A and B antigens on red blood cells.
IgM is the largest antibody in the human blood. IgM forms polymers where multiple immunoglobulins are covalently linked together with disulfide
bonds, mostly as a pentamer but also as a hexamer. IgM has a molecular mass of approximately 900 kD (in its pentamer form). Because each
monomer has two antigen binding sites, a pentameric IgM has 10 binding sites. Typically however, IgM cannot bind 10 antigens at the same
time because the large size of most antigens hinders binding to nearby sites. Due to its polymeric nature, IgM possesses high avidity, and is
particularly effective in germline cells; the gene segment encoding the μ constant region of the heavy chain is positioned first among other
constant region gene segments. For this reason, IgM is the first immunoglobulin expressed by mature B-cells. IgM antibodies appear early in
the course of an infection and usually reappear, to a lesser extent, after further exposure. Demonstrating IgM antibodies in a patient’s serum
indicates recent infection.
Anti-IgM reacts with surface immunoglobulin M μ chains. IgM is one of the predominant surface immunoglobulins on B-lymphocytes. This
antibody is useful when identifying lymphomas, plasmacytomas, and B-cell lineage-derived Hodgkin lymphomas.
Immunoglobulin, Heavy and Light Chain
IgM
IgA
IgG
IgD
Kappa
Lambda
Secretory Meningioma
+
+
-
-
Cutaneous Lymphoma
-
-
-
-
+/-
-/+
Myeloma
-/+
+
+
-/+
+/-
-/+
Diffuse LBCL
+
-
+
-
+/-
-/+
Marginal Zone Lymphoma
+
-
-
-/+
+/-
-/+
SLL/CLL
+
-
-
+
+/-
-/+
Reference
1. Arnold A, et al. New Eng J Med. 1983; 309:1593-1599.
2. Hertel BF, et al. New Eng J Med. 1980; 302:1293-1297.
3. Taylor CR. Arch Path Lab Med. 1978; 102:113-121.
4. Warnake R, et al. Masson Publishing USA. 1981. pp 203-221.
5. de Boer CJ, et al. Ann Oncol. 1997; 8 Suppl 2:109-17.
6. Kojima M, et al. APMIS. 2002 Dec; 110(12):875-80.
7. Pambuccian SE, et al. Am J Surg Pathol. 1997 Feb; 21(2):179-86.
149
