Antibodies for
Immunohistochemistry
CD23 (1B12)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45178 IMPATH CD23 RTU M (1B12)
50 Tests
44229 CD23 RTU M (1B12)
7 ml Ready To Use
44480 CD23 0,1 M (1B12)
100 µl liquid Concentrated
44481 CD23 1 M (1B12)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/K
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-CD23 is a B-cell antibody that is useful in differentiating between small lymphocytic lymphoma/chronic lymphocytic leukemia (anti-CD23
immunoreactive) from mantle cell lymphoma and follicular lymphoma (immunonegative). This antibody reacts with the antigen that is found on
a subpopulation of peripheral blood cells, B-lymphocytes, and on EBV transformed B-lymphoblastoid cell lines. Anti-CD23 recognizes follicular
dendritic cell meshwork and follicular dendritic sarcoma.
B-cell Lymphomas
CD23
TCL1
CD20
BCL2
BCL6
CD10 MUM1 Cyclin D1 CD5
TRAcP
Follicular
-
+
+
+
+
+
-
-
-
-
CLL/SLL
+
+
+
+
-
-
+
-
+
-
Mantle Cell
-
+
+
+
-
-
-/+
+
+
-
Marginal Zone
-
-
+
+
-
-
+
-
-
+/-
Lymphoplasmacytic
-
+
+
+
-
-
+
-
-
-
Diffuse Large Cell
-
+
+
+
+
-/+
+
-
-/+
-
Burkitt
-
+
+
-
+
+
-
-
-
-
Hairy Cell Leukemia
-
+
+
+
-
-
+(weak)/-
-
+
Reference
1. Kaiserlian D, et al. Immunology. 1993; 80:90-95.
2. Aubry JP, et al. Oxford Univ Press-Oxford, NY, Tokyo. 1987; 417-419.
3. Pallesen G. Oxford Univ Press-Oxford, NY, Tokyo 1987; 383-386.
4. Pezzella F, et al. Br j Haematol. 2000 Feb; 108(2):369-76.
5. Kurtin PJ, et al. Am J Clin Pathol. 1999 Sep; 112(3):319-29.
6. West RB, et al. Am J Clin Pathol. 2002 Apr; 117(4):636-43.
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