Advanced Solutions
for Advanced Pathology
CD25 (4C9)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45129 IMPATH CD25 RTU M (4C9)
50 Tests
44230 CD25 (Interleukin-2 Receptor) RTU M (4C9)
7 ml Ready To Use
44482 CD25 (Interleukin-2 Receptor) 0,1 M (4C9)
100 µl liquid Concentrated
44483 CD25 (Interleukin-2 Receptor) 1 M (4C9)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Lesions of Mastocytosis
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2b
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
According to the World Health Organization classification system, the major diagnostic criterion for bone marrow involvement by systemic
mastocytosis (SM) is the presence of dense aggregates (>15 cells) of mast cells. Expression of CD25, a low-affinity receptor for interleukin-2 (IL-
2), affords a reliable diagnostic means for distinguishing neoplastic mast cell aggregates from reactive proliferations, and has therefore recently
become a criterion for the diagnosis of SM combined with CD2 positivity. Anti-CD25 is therefore suggested as the “marker of choice” for cases
of suspected systematic mastocytosis. Therefore, aberrant staining of mast cell clusters by anti-CD25 in tissue is essentially diagnostic of SM.
Anti-CD25 has also been useful in identifying mast cells in skin biopsies in the setting of urticaria pigmentosa, which is associated with SM.
B-cell Lymphomas
CD25
CD45
CD20
CD79a
BCL2
T-bet
TRAcP Annexin A1 CD43
Hairy Cell Leukemia
+
+
+
+
+
+
+
+
-
T-cell Lymphomas
CD25
CD2
CD3
CD4
CD5
CD7
CD8 CD45RO PD-1 Granzyme
B
Angioimmunoblastic
+
+
+
+
+
+
-
+
+
-
Lymphoblastic
+
+/-
+
+/-
+
+
+/-
+
-
+/-
Subcutaneous
Panniculitis-Like
-
+
+
-
+
+
+/-
+
-
+
NK
+
+
+
-
-
-/+
-
+
-
+
Cutaneous
-
+
+
+
-
+
-
-
-/+
+
Peripheral, NOS
+
+
+
+/-
+/-
+/-
-/+
+
-
-/+
Mycosis Fungoides
+
+
+
+
+
-
-
+
-
+/-
Mastocytosis
CD25
Tryptase
CD117
CD163
CD2
Systemic Mastocytosis
+
+
+
-
+
Mastocytic Leukemia
+
+
+
-
+
Reactive Mast Cells
-
+
+
+
-
Reference
1. Hahn HP, Hornick JL. Am J Surg Pathol. 2007 Nov; 31(11):1669-76.
2. Hollmann TJ, Brenn T, Hornick JL. Am J Surg Pathol. 2008 Jan; 32(1):139-45.
3. Miracco C, et al. Oncol Rep. 2007 Nov; 18(5):1115-22.
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