Antibodies for
Immunohistochemistry
CD30 (Ber-H2)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45180 IMPATH CD30 RTU M (Ber-H2)
50 Tests
44232 CD30 RTU M (Ber-H2)
7 ml Ready To Use
44486 CD30 0,1 M (Ber-H2)
100 µl liquid Concentrated
44487 CD30 1 M (Ber-H2)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Classic Hodgkin Lymphoma,
Lymphoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-CD30 detects a formalin-resistant epitope that is expressed by Reed-Sternberg cells in classic Hodgkin lymphoma, the majority of anaplastic
large cell lymphomas, primary cutaneous CD30 positive T-cell lymphoproliferative disorders, and in embryonal carcinomas. Occasionally
diffuse large B-cell lymphoma stains with this antibody. This antibody also stains plasma cells in paraffin-embedded tissue. However, reactive
immunoblasts are immunoreactive with this antibody. The staining pattern of anti-CD30 in lymphoma or embryonal carcinoma is different, with
the former being membranous and exhibiting Golgi zone accentuation in location, and the latter being membranous only.
Hodgkin vs. Non-Hodgkin Lymphomas
CD30 CD79a CD15 Fascin Granzyme
B
BCL6
PU.1
MUM1 ALK-1
EMA
Hodgkin Lymphoma,
Classic
+
-
+
+
-
-
-
+
-
-
Hodgkin Lymphoma,
Nodular Lymphocyte
Predominant
-
+
-
-
-
+
+
-/+
-
+
T-cell Rich LBCL
-
+
-
-
-
+
-
+
-
-
Anaplastic Large Cell
Lymphoma
+
-
-
-
+
+/-
-
-
+
+
Germ Cell Tumors
CD30
Oct-4
AFP Vimentin EMA Inhibin
hPL Glypican-3 CD117 PLAP
Seminoma
-
+
-
+
-
-
-
-
+
+
Embryonal Carcinoma
+
+
-
-
-
-
-
-
-
+
Choriocarcinoma
-
-
-
-/+
+
-
+
+
-
+
Yolk Sac Tumor
-
-
+
-
-
-
-
+
-
+
Granulosa Cell Tumor
-
-
-
+
-
+
-
-
-
-
Hypercalcaemic Small Cell
Carcinoma
-
-
-
-
+
-
-
-
-
-
Reference
1. Schwarting R, et al. Blood. 1989; 74:1678-1689.
2. Fonatsch C, et al. Genomics. 1992; 14:825-826.
3. Piris J, et al. Histopathology. 1990; 17:211-218.
4. George DH, et al. Am J Surg Pathol. 2003 Apr; 27(4):487-93.
5. Hedvat CV, et al. Hum Pathol. 2002 Oct; 33(10):968-74.
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