Advanced Solutions
for Advanced Pathology
CD31 (JC70)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45181 IMPATH CD31 RTU M (JC70)
50 Tests
44233 CD31 RTU M (JC70)
7 ml Ready To Use
44488 CD31 0,1 M (JC70)
100 µl liquid Concentrated
44489 CD31 1 M (JC70)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental
studies as well as for bone marrow transplantation. Endothelial cells also express this marker, therefore, antibodies to CD31 have been used as a
tool to identify the vascular origin of neoplasms. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining
of nonvascular tumors (excluding hematopoietic neoplasms) has not been observed with this antibody.
Skin: Spindle Cell Tumors
CD31
FLI-1 Factor VIII
HHV-8 SM Actin MS Actin Collagen
IV
D2-40 NGFR CD10
Squamous Cell Carcinoma
-
-
-
-
-
-
-
+
-
-
Spindle Cell Melanoma
-
+
-
-
-
-
-
+
+
-
Atypical Fibroxanthomas
-
-
-
-
+
+
-
-
-
+
DF-SP
-
-
-
-
-
-
-
-
+
+/-
DF-FH
-
-
-
-
-
-
-
-
-
+
Peripheral Nerve Sheath
-
-
-
-
-
+
-
+
-
-
Smooth Muscle
-
-
-
-
+
+
-
-
-
-
Angiosarcoma
+
+
+
-
-
-
+/-
+/-
-
-
Glomus Tumor
-
-
-
-
+
+
+
-
-
-
Solitary Fibrous Tumor
-
-/+
-
-
-
-
-
-
-
-
Hemangioma
+
+
+
-
+
-
+
-
-
-
Hemangioendothelioma
+
+
-
-
-
-
-
-
-
-
Kaposi's Sarcoma
+/-
+
+
+
+
-
+/-
+
-
-
Renal Cell Carcinoma vs. Hemangioblastoma
CD31
D2-40
FLI-1
CK Cocktail
CD10
Adipophilin
Metastatic RCC
-
-
-
+
+
-
Hemangioblastoma
+
+
+
-
-
+
Reference
1. Parums DV, et al. J Clin Pathol. 1990 Sep; 43(9):752-7.
2. De Young BR, et al. Applied Immunohistochemistry. 1993; 1:97-100.
3. Alles JU, Bosslet K. J Histochem Cytochem. 1986 Feb; 34(2):209-14.
4. Alexander-Sefre F, et al. J Clin Pathol. 2003 Oct; 56(10):786-8.
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