Advanced Solutions
for Advanced Pathology
CD34 (QBEnd/10)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45131 IMPATH CD34 RTU M (QBEnd/10)
50 Tests
44235 CD34 RTU M (QBEnd/10)
7 ml Ready To Use
44492 CD34 0,1 M (QBEnd/10)
100 µl liquid Concentrated
44493 CD34 1 M (QBEnd/10)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Placenta, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) for AP 2-step
Polymer (Universal) 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-CD34 recognizes a cell surface antigen of approximately 110 kD that is expressed selectively on human hematopoietic progenitor cells,
including myeloid and lymphoid lineage progenitors. It is a marker of choice for identifying and counting the blasts in acute myeloid leukemia.
In addition, this marker is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression
is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule in greater amounts than
non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi’s sarcoma, but shows low specificity.
Soft Tissue Sarcoma
CD34 CK Cocktail
EMA MS Actin TLE-1
S-100
CD99
CD56 Calretinin
Epithelioid Sarcoma
+
+
+
-/+
-
-
-
-
-
Myxoid Chondrosarcoma
-/+
-
-
-
-
+/-
-
+
Synovial Sarcoma
-
+
+
-
+
-
+/-
+
+/-
Skin: Spindle Cell Tumors
CD34
FLI-1 GLUT1
BG8 Factor VIII
HHV-8 CK 8 & 18 NGFR Collagen
IV
CD99
DFSP
+
-
-
-
-
-
-
+
-
-
Angiosarcoma
+
+
-
-
+
-
-
-
+/-
-
Solitary Fibrous Tumor
+
-/+
-
-
-
-
-
-
-
+/-
Hemangioma
+
+
+
+
+
-
-
-
+
-
Hemangioendothelioma
+
+
-
-
-
-
+
-
-
-
Kaposi's Sarcoma
+
+
-
-
+
+
-
-
+/-
-
Epithelioid Cell Neoplasms
CD34
INI-1
EMA
FLI-1 Desmin GLUT1 Claudin 1 MS Actin SM Actin CD56
Epithelioid Sarcoma
+
+
+
-
+
+
+
+
-
-
Epithelioid Angiosarcoma
+
+
-
+
-
GIST
+
-
-
-
-
-
-
-
-
Reference
1. Civin CL, et al. London Academic Press. 1989; 818-825.
2. Fina L, et al. Blood. 1990; 75:2417-2426.
3. Sankey EA, et al. J Pathol. 1990; 43:752-757.
4. Ramani P, et al. Histopathology. 1990; 17:237-242.
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