Advanced Solutions
for Advanced Pathology
HGAL (MRQ-49)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45311 IMPATH HGAL RTU M (MRQ-49)
50 Tests
44311 HGAL RTU M (MRQ-49)
7 ml Ready To Use
44644 HGAL 0,1 M (MRQ-49)
100 µl liquid Concentrated
44645 HGAL 1 M (MRQ-49)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Lymph node, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2a
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Human germinal center–associated lymphoma (HGAL) protein is specifically expressed in the cytoplasm of germinal center B-cells, but is
absent in mantle and marginal zone B-cells and in the interfollicular and paracortical regions in normal tonsils and lymph nodes. Its high degree
of specificity for germinal center B-cells makes anti-HGAL an ideal marker for the detection of germinal center-derived B-cell lymphomas.
Anti-HGAL has the highest overall sensitivity of detecting follicular lymphoma (FL) and in detecting the interfollicular and diffuse components of
FL compared with antibodies against BCL2, LMO2, CD10, and BCL6. The addition of anti-HGAL to the immunohistochemical panel is beneficial
in the work-up of nodal and extranodal B-cell lymphomas and the efficacy of anti-HGAL in detecting the follicular, interfollicular, and diffuse
components of FL is of particular value in the setting of variant immunoarchitectural patterns.
Mature B-cell Lymphomas
HGAL
LMO2
CD20
CD5
CD23
CD10
BCL2
Follicular Lymphoma
+
+
+
-
-
+/-
+/-
Diffuse Large B-cell
Lymphoma
+
+
+
-/+
-
+/-
+
Small Lymphocytic
Lymphoma
-
-
+
+
+
-
+
Mantle Cell Lymphoma
-
-
+
+
-
-
+
Marginal Zone Lymphoma
-
-
+
-
-
-
+
Reference
1. Natkunam Y, et al. Blood. 2005; 105:3979–3986.
2. Natkunam Y, et al. Blood. 2007; 109:298-305.
3. Younes SF, et al. Am J Surg Pathol. 2010; 34:1266-1276.
4. Higgins RA, et al. Arch Pathol Lab Med. 2008; 132:441-446.
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