Advanced Solutions
for Advanced Pathology
HMB-45 + Mart-1 (Melan A) + Tyrosinase
(HMB-45 + A103 + T311)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45318 IMPATH HMB-45&MART-1 RTU M (HMB-45&MelanA)
50 Tests
44333 HMB-45 + MART-1 (MelanA) RTU M (HMB-45, A103, T311)
7 ml Ready To Use
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Melanoma, Skin
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-Melanoma (HMB-45) identifies immature melanosomes. MART-1 (also known as Melan A) is a melanocyte differentiation antigen. It is
present in melanocytes of normal skin and retina, nevi and in more than 85% of melanomas. Tyrosinase is an enzyme integral in the process
of melanin synthesis, and found in 85% to 90% of malignant melanomas. Given these statistics, this cocktail is ideally suited to detection of
melanomas and melanocytic lesions.
Reference
1. Orchard G. Br J Biomed Sci. 2002; 59(4):196-202.
2. Gupta D, et al. Am J Surg Pathol. 2002 Nov; 26(11):1450-7.
3. Prasad ML, et al. Am J Surg Pathol. 2001 Jun; 25(6):782-7.
4. de Vries TJ, et al. J Pathol. 2001 Jan; 193(1):13-20.
5. Yaziji H, Gown AM. In J Surg Pathol. 2003 Jan; 11(1):11-5.
6. Sindham VB, et al. BMC Cancer. 2003 May 7; 3(1):15.
7. Perez RP, et al. Hum Pathol. 2000 Nov; 31(11):1381-8.
8. Hoang MP, et al. J Cutan Pathol. 2001 Sep; 28(8):400-6.
9. Baidsen BL, et al. Am J Surg Pathol. 2000 Aug; 24(8):1140-6.
10. Vaggelli L, et al. Tumori. 200 Jul-Aug; 86(4):346-8.
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