Antibodies for
Immunohistochemistry
Human Placental Lactogen (hPL) (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45312 IMPATH Hpl RTU R (Poly)
50 Tests
44314 hPL RTU R (Poly)
7 ml Ready To Use
44650 hPL 0,1 R (Poly)
100 µl liquid Concentrated
44651 hPL 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Placenta
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Human placental lactogen (hPL), also previously known as ‘human chorionic somatomammotropin’, is a 22 kD protein with partial homology to
growth hormone. hPL is first detectable in the maternal serum in the fifth week of gestation and reaches a plateau by the thirty-fourth week. hPL
has been demonstrated by immunochemistry in the syncytiotrophoblastic cells of choriocarcinoma. A rare variant of trophoblastic tumor has
been reported in the testis with resemblance to uterine placental site trophoblastic tumor. It consists purely of intermediate trophoblasts which
are diffusely positive for hPL and focally for ß-hCG.
Gonads: Germ Cell Tumors vs. Somatic Adenocarcinoma
hPL
Oct-4
AFP Vimentin EMA Inhibin CD30 Glypican-3 CD117 PLAP
Seminoma
-
+
-
+
-
-
-
-
+
+
Embryonal Carcinoma
-
+
-
-
-
-
+
-
-
+
Choriocarcinoma
+
-
-
-/+
+
-
-
+
-
+
Yolk Sac Tumor
-
-
+
-
-
-
-
+
-
+
Somatic Carcinoma
-
-
-
-
+
-
-
-
-
-
Granulosa Cell Tumor
-
-
-
+
-
+
-
-
-
Hypercalcaemic Small Cell
Carcinoma
-
-
-
-
+
-
-
-
-
-
Placental Trophoblastic Cells
1st Trimester
2nd Trimester
3rd Trimester
hCG
hPL
hCG
hPL
hCG
hPL
Cytotrophoblast
-
-
-
-
-
-
Intermediate Trophoblast
1-24%
25-49%
- /+
50-74%
1-24%
1-49%
Syncytiotrophoblast
>75%
1-24%
25-49%
50-74%
1-24%
>75%
Trophoblastic Proliferations
hPL
p57
hCG
PLAP
CK, OSCAR
Vimentin
Partial Mole
Weak, diffuse
+
Weak, diffuse
+
Strong, diffuse
-
Complete Mole
Weak, focal
-
Strong, diffuse
Weak, focal
Strong, diffuse
-
Choriocarcinoma
Weak, focal
-
Strong, diffuse
Weak, focal
Strong, diffuse
-/+
Placental Site Tumor
Strong, diffuse
Strong, focal
Strong, diffuse Strong, diffuse Strong, diffuse
Reference
1. Shih IM, Kurman RJ. Am J Surg Pathol. 2004 Sep; 28(9):1177-83.
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